Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diversity of Protists IV01:27

Diversity of Protists IV

Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
Diversity of Protists III01:27

Diversity of Protists III

Rhizaria are a diverse group of unicellular protists characterized by their threadlike cytoplasmic extensions known as pseudopodia. These structures aid in both locomotion and feeding, giving Rhizaria an amoeboid appearance. Their amoeboid morphology once led to taxonomic confusion, but molecular phylogenetics has clarified their evolutionary placement and emphasized their shared use of pseudopodia despite divergent lineages.This clade comprises diverse lineages such as Chlorarachniophyta,...
Diversity of Protists II01:27

Diversity of Protists II

Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
Diversity of Protists I01:15

Diversity of Protists I

Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
Stratified Sampling Method01:16

Stratified Sampling Method

Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
To choose a stratified sample, divide the population into groups called strata and then take a...
Marine Microbial Ecology01:30

Marine Microbial Ecology

Marine microbial ecosystems are shaped by distinct physicochemical limits, including high salinity, low nutrient availability, and fluctuating oxygen levels. These conditions favor smaller microbial cell sizes, which maximize their surface-to-volume ratio for efficient nutrient uptake.Microbial activity and community composition are closely linked to biogeochemical cycles, particularly in dynamic environments like estuaries, where halotolerant microbes thrive in response to variable salinity...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.

Molecular ecology resources·2026
Same author

An updated patent review of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) inhibitors (2020-present).

Expert opinion on therapeutic patents·2026
Same author

Structure-Based Design, Synthesis, and Evaluation of Novel Ponatinib Derivatives With a Significantly Altered Selectivity Profile.

ChemMedChem·2026
Same author

Benzothiophene nucleus in target-directed anti-cancer drug discovery.

European journal of medicinal chemistry·2026
Same author

Predicting sediment ecological state from metagenomes shows equal performance for taxonomic and functional features.

Marine environmental research·2026
Same author

Protistan Plankton Responses to Variable Light and Upwelling in the Peruvian Humboldt Current System: Insights Into Community Dynamics Under Environmental Change.

Ecology and evolution·2026
Same journal

Host-selective PMA-PCR enhances bacterial diversity recovery and lowers detection limits in plant microbiome profiling.

FEMS microbiology ecology·2026
Same journal

Versatile microbial community responsible for nitrate turnover in a carbonate aquifer in southwest Germany.

FEMS microbiology ecology·2026
Same journal

Geochemistry shapes microbial diversity and selected functional traits in flowback and produced waters from hydraulically fractured formations.

FEMS microbiology ecology·2026
Same journal

Desiccation, but not shading, substantially shifts the microbial community composition of benthic microbial mats in meltwater ponds near Bratina Island, Antarctica.

FEMS microbiology ecology·2026
Same journal

Soil microbial ecology and microbiome-metabolite linkages improve understanding of ecosystem states along terrestrial-aquatic interfaces.

FEMS microbiology ecology·2026
Same journal

Electrogenic CH4 oxidation on a bioanode: putative extracellular electron transport system in Methylobacter sp.

FEMS microbiology ecology·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

Automatic Image Processing to Determine the Community Size Structure of Riverine Macroinvertebrates
08:56

Automatic Image Processing to Determine the Community Size Structure of Riverine Macroinvertebrates

Published on: January 13, 2023

Sample pooling obscures diversity patterns in intertidal ciliate community composition and structure.

Matthias Engel1, Anke Behnke, Sara Bauerfeld

  • 1Department of Ecology, University of Kaiserslautern, Kaiserslautern, Germany.

FEMS Microbiology Ecology
|November 19, 2011
PubMed
Summary
This summary is machine-generated.

Sample pooling obscures ciliate diversity in intertidal sediments. However, pooling may still reveal key ecological players by focusing on abundant taxa, despite altering community composition profiles.

More Related Videos

Utilizing Soil Density Fractionation to Separate Distinct Soil Carbon Pools
09:19

Utilizing Soil Density Fractionation to Separate Distinct Soil Carbon Pools

Published on: December 16, 2022

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders
08:57

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders

Published on: August 3, 2016

Related Experiment Videos

Last Updated: May 27, 2026

Automatic Image Processing to Determine the Community Size Structure of Riverine Macroinvertebrates
08:56

Automatic Image Processing to Determine the Community Size Structure of Riverine Macroinvertebrates

Published on: January 13, 2023

Utilizing Soil Density Fractionation to Separate Distinct Soil Carbon Pools
09:19

Utilizing Soil Density Fractionation to Separate Distinct Soil Carbon Pools

Published on: December 16, 2022

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders
08:57

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders

Published on: August 3, 2016

Area of Science:

  • Marine Ecology
  • Microbial Ecology
  • Molecular Ecology

Background:

  • Intertidal sediments harbor diverse microbial communities.
  • Accurate assessment of microbial community structure is crucial for ecological studies.
  • Molecular techniques are widely used to profile microbial eukaryotes.

Purpose of the Study:

  • To evaluate the impact of sample pooling on ciliate community structure and composition.
  • To compare molecular profiles from individual replicates versus pooled samples.
  • To determine if sample pooling affects the detection of ecologically significant taxa.

Main Methods:

  • Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis of SSU rRNA.
  • Analysis of ciliate community profiles from intertidal sediment samples.
  • Comparison of data from nine biological replicates and pooled samples.

Main Results:

  • Pooled samples showed significantly different community composition compared to cumulative individual replicates.
  • Sample pooling obscured diversity patterns in ciliate communities.
  • Differences were less pronounced when analyzing community structure versus composition.
  • Abundant taxa were generally consistent between pooled and individual samples.

Conclusions:

  • Sample pooling can obscure microbial eukaryote diversity, including ciliates.
  • Pooling may be suitable for identifying dominant taxa that drive ecosystem processes.
  • Researchers should carefully consider the implications of sample pooling for their specific study aims.