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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: May 27, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Automated digital microfluidic sample preparation for next-generation DNA sequencing.

Hanyoup Kim¹, Michael S Bartsch, Ronald F Renzi

¹Sandia National Laboratories, Livermore, CA 94550, USA.

Journal of Laboratory Automation

|November 19, 2011

Summary

This summary is machine-generated.

This study presents a digital microfluidic platform for automated next-generation sequencing (NGS) library preparation. This automated system enhances speed and efficiency for pathogen identification in biodefense and public health applications.

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Last Updated: May 27, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Area of Science:

Biotechnology
Microfluidics
Genomics

Background:

Next-generation sequencing (NGS) is vital for pathogen identification but hindered by slow, manual DNA sample preparation.
Automated methods are needed for time-critical biodefense and public health applications.

Purpose of the Study:

To develop an automated digital microfluidic (DMF) platform for integrated NGS library preparation.
To demonstrate the platform's capability for automated liquid handling and sample manipulation.

Main Methods:

A novel capillary interface was developed for precise liquid transfer between DMF devices and external modules.
The platform integrated continuous-flow and droplet-based manipulations for automated fraction collection and magnetic bead-based cleanup.
Contactless conductivity detection enabled closed-loop automated fraction collection.

Main Results:

Demonstrated automated fraction collection of target analytes using the DMF platform and capillary interface.
Achieved an average DNA recovery efficiency of 80%±4.8% for buffer exchange and sample cleanup using magnetic beads.

Conclusions:

The DMF hub platform with a capillary interface effectively automates key NGS library preparation steps.
This integrated system offers a fast, efficient, and reliable solution for automated DNA sample preparation, advancing biodefense and public health applications.