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Published on: January 17, 2015
Selection of lead antibodies from naive ribosome display antibody libraries
1Department of Antibody Discovery and Protein Engineering, MedImmune Limited, Cambridge, UK. ravnp@medimmune.com
A large antibody fragment library (>10(12)) has been generated in ribosome display format. The library was constructed in a two-step process. First, variable (V) genes were isolated from human B cells from a panel of 14 donors and cloned into designated ribosome display vectors to create a gene bank. Second, RD-VH and RD-VL genes from individual immunoglobulin families were combined in vitro resulting in 112 scFv ribosome display sub-libraries. These were subsequently pooled to form a master library.This library was used to isolate a panel of antibodies to the IL4 receptor by three rounds of selections on a soluble target.
A large antibody fragment library (>10(12)) has been generated in ribosome display format. The library was constructed in a two-step process. First, variable (V) genes were isolated from human B cells from a panel of 14 donors and cloned into designated ribosome display vectors to create a gene bank. Second, RD-VH and RD-VL genes from individual immunoglobulin families were combined in vitro resulting in 112 scFv ribosome display sub-libraries. These were subsequently pooled to form a master library.This library was used to isolate a panel of antibodies to the IL4 receptor by three rounds of selections on a soluble target.
