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Updated: May 27, 2026

Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Ribosome display for the selection of Sac7d scaffolds
Barbara Mouratou1, Ghislaine Béhar, Lauranne Paillard-Laurance
1Unité de Biotechnologie, Biocatalyse et Biorégulation, UMR6204 CNRS, Université de Nantes, Nantes, France.
Abstract:
Combinatorial libraries of Sac7d have proved to be a valuable source of proteins with favorable biophysical properties and novel ligand specificities, so-called Nanofitins. Thus, Sac7d represents a promising scaffold alternative to antibodies for biotechnological and potentially clinical applications. We describe here the methodology for the construction of a library of Sac7d and its use for selection by ribosome display.
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