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Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
Published on: October 3, 2019
In vitro selection of unnatural cyclic peptide libraries via mRNA display
Zhong Ma1, Matthew C T Hartman
1Department of Chemistry, Virginia Commonwealth University, Richmond, VA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 19, 2011
Abstract:
The ribosomal synthesis of drug-like peptides containing unnatural amino acids is possible due to the broad substrate specificity of the ribosome. In this protocol, a reconstituted Escherichia coli ribosomal translation system (PURE) is adapted to incorporate unnatural amino acids into mRNA-displayed peptide libraries, which are used in in vitro selection.
