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Updated: May 27, 2026

11:46
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Published on: May 26, 2014
A FlAsH-based cross-linker to study protein interactions in living cells
Anna Rutkowska1, Christian H Haering, Carsten Schultz
1Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Angewandte Chemie (International Ed. in English)
|November 19, 2011
Abstract:
As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).
