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Related Experiment Videos

Techniques in plant molecular biology--progress and problems.

R Walden1, J Schell

  • 1Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.

European Journal of Biochemistry
|September 24, 1990
PubMed
Summary

Efficient gene transfer methods are crucial for plant molecular biology advancements. This review covers Agrobacterium and viral vectors for DNA expression, gene analysis, and isolation, discussing current system limitations.

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Fusion of Agrobacterium and E. coli spheroplasts with Nicotiana tabacum protoplasts - Direct gene transfer from microorganism to higher plant.

Plant cell reports·2013

Area of Science:

  • Plant molecular biology
  • Genetics
  • Biotechnology

Background:

  • Efficient introduction of foreign DNA into plant cells is fundamental for progress in plant molecular biology.
  • Gene transfer can be achieved through direct DNA uptake or via the soil bacterium Agrobacterium tumefaciens.
  • Development of gene-transfer vectors, including those based on Agrobacterium and plant viruses, has been pivotal.

Purpose of the Study:

  • To review the advancements in plant gene-transfer technologies.
  • To discuss the applications of these technologies in plant molecular biology research.
  • To highlight the limitations of current experimental systems.

Main Methods:

  • Utilizing Agrobacterium-mediated gene transfer.
  • Employing plant virus-based vectors for gene transfer.

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  • Reviewing existing literature on gene transfer techniques and applications.

    • Main Results:

    • Gene transfer vectors enable the expression of foreign DNA in plants.
    • These vectors facilitate functional analysis of plant DNA sequences.
    • They are instrumental in studying viral replication, cell-to-cell spread, and transposition.
    • Gene transfer vectors can be used to isolate genes not easily accessible by conventional methods.

    Conclusions:

    • Versatile gene-transfer vectors have significantly advanced plant molecular biology.
    • These tools allow for diverse applications, from gene expression to gene isolation.
    • Further development is needed to overcome the limitations of current experimental systems.