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Idiotypic interactions between normal human polyspecific IgG and natural IgM antibodies.
F Rossi1, B Guilbert, C Tonnelle
1Unité d'Immunopathologie et INSERM U28, Hôpital Broussais, Paris, France.
European Journal of Immunology
|September 1, 1990
Summary
Pooled normal human polyspecific immunoglobulin G (IVIg) contains antibodies that interact with natural IgM antibodies. This suggests IVIg may influence the B cell repertoire and potentially modulate autoimmune disease activity.
Area of Science:
- Immunology
- Autoimmunity
- Antibody Engineering
Background:
- Pooled normal human polyspecific immunoglobulin G (IVIg) contains anti-idiotypes against autoantibodies and IgG autoantibodies.
- Previous research suggests IVIg interacts with autoantibodies, but its interaction with natural IgM antibodies is less understood.
Purpose of the Study:
- To investigate the interaction between IVIg and natural IgM antibodies.
- To determine if IVIg contains anti-idiotypes that bind to natural IgM antibodies.
Main Methods:
- Testing IgM secreted by B lymphoid cell lines and EBV-transformed cord B cells for binding to IVIg F(ab')2 fragments.
- Inhibiting the binding of polyreactive IgM to 2,4,6-trinitrophenyl (TNP) using IVIg F(ab')2 fragments.
Main Results:
- 64% of IgM from healthy elderly donors' B cell lines and 18% from cord B cells bound to IVIg F(ab')2 fragments.
- IVIg F(ab')2 fragments inhibited the binding of a polyreactive IgM to TNP, indicating anti-idiotype activity against the antibody-combining site.
- IgM interaction with IVIg idiotypes was independent of polyreactivity.
Conclusions:
- IVIg contains anti-idiotypes that react with natural IgM antibodies.
- These findings suggest IVIg may modulate the B cell repertoire and have implications for the treatment of autoimmune diseases.