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[Simultaneous determination of six flavonoids in Hyperici Japonici Herba by HPCE-DAD]
Ke Han1, Xunhang Liu, Qinan Wu
1Pharmnacology College of Nanjing University of Chinese Medicine, Nanjing 210046, China. winner2243@sohu.com
Objective:
To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin, isoquercitrin, hyperoside, quercitrin, kaempferol and quercetin in Hyperici Japonici Herba.
Method:
Based on the mode of capillary zone electrophoresis, 40 mmol x L(-1) borax was used as buffer solution (pH 8.62), uncoated fused silica capillary (56 cm x 64.5 cm x 75 microm) was used, separation voltage was 25 kV, detection wavelength was at 206 nm, column temperature was maintained at 25 degrees C, and sample was injected at 50 mbar, 8 s.
Result:
Six flavonoids showed good linearity (r > 0.9953) in the range of the tested concentration, the average recoveries of the method were between 98.8%-102.9%.
Conclusion:
The method is simple, accurate and reproducible, and can be used for quality control of Hyperici Japonici Herba.