Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lectin binding patterns in developing canine retina.

H E Whiteley1, J R Scott

  • 1Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.

Experimental Eye Research
|October 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictors of interferon-gamma release assay results and their association with COVID-19 infection outcomes.

IJTLD open·2024
Same author

Correction to: Characterization of host response, resorption, and strength properties, and performance in the presence of bacteria for fully absorbable biomaterials for soft tissue repair.

Hernia : the journal of hernias and abdominal wall surgery·2024
Same author

Optogenetic stimulation of striatal patches modifies habit formation and inhibits dopamine release.

Scientific reports·2021
Same author

What are the immunological and clinical implications of microchimerism?

BJOG : an international journal of obstetrics and gynaecology·2018
Same author

Safety of eliminating postpartum magnesium sulphate: intriguing but not yet proven.

BJOG : an international journal of obstetrics and gynaecology·2018
Same author

Characterization of host response, resorption, and strength properties, and performance in the presence of bacteria for fully absorbable biomaterials for soft tissue repair.

Hernia : the journal of hernias and abdominal wall surgery·2017
Same journal

Caffeine suppresses inflammation and subretinal fibrosis through modulation of the thrombospondin-1-Bim axis.

Experimental eye research·2026
Same journal

Single-cell transcriptomic profiling reveals functional heterogeneity and regulatory programs of neutrophils in experimental autoimmune uveitis.

Experimental eye research·2026
Same journal

Three-Dimensional Platform-Dependent Modulation of Stemness and EMT-Associated Gene Expression in Primary and Metastatic Uveal Melanoma Models.

Experimental eye research·2026
Same journal

Early Proteomic and Metabolic Signatures of Liver and Eye in OAT-Deficient Mice.

Experimental eye research·2026
Same journal

Inhibition of STAT6 signaling by rebamipide attenuates IL-4- and IL-13-induced eotaxin expression in corneal fibroblasts.

Experimental eye research·2026
Same journal

Translational animal model for genetic predisposition to anophthalmia/microphthalmia.

Experimental eye research·2026
See all related articles

This study reveals that canine retinal glycoproteins change with age, showing differential expression across retinal layers during development. These changes are regulated by time and location.

Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Glycobiology

Background:

  • The canine retina's complex structure and cellular composition are crucial for vision.
  • Understanding the molecular composition of the developing retina is key to understanding visual system development and disease.

Purpose of the Study:

  • To investigate the temporal and topographical expression of glycoproteins in the developing canine retina.
  • To identify specific lectin binding patterns in different retinal layers during development.

Main Methods:

  • Canine retinas at various developmental stages were fixed and examined using a panel of biotinylated lectins.
  • Lectin binding affinities were analyzed across different retinal layers, including the retinal pigment epithelium, photoreceptor segments, plexiform layers, and ganglion cells.

Related Experiment Videos

Main Results:

  • Concanavalin A (ConA) and Wheat Germ Agglutinin (WGA) bound to all retinal layers, with increased binding intensity in older animals.
  • Specific lectins like Soybean Agglutinin (S-WGA), Dolichos Biflorus Agglutinin (DBA), and Peanut Agglutinin (PNA) showed differential binding to photoreceptor inner and outer segments and retinal pigment epithelium.
  • Ricinus Communis Agglutinin (RCA) intensely labeled retinal vessels, as did PNA after neuraminidase treatment, indicating specific carbohydrate structures.

Conclusions:

  • The developing canine retina exhibits developmentally regulated and spatially distinct patterns of glycoprotein expression.
  • Lectin binding patterns suggest that glycoproteins play significant roles in retinal development, differentiation, and structural organization.