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Related Concept Videos

Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...

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Updated: May 27, 2026

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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[FTIR microspectroscopy and its progress in application].

Xiao-Ting Li1, Da-Zhou Zhu, Li-Gang Pan

  • 1Shanghai Jiao Tong University School of Agriculture and Biology, Shanghai 200240, China. lxt830407@yahoo.com.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|November 22, 2011
PubMed
Summary
This summary is machine-generated.

Fourier-transform infrared (FTIR) microspectroscopy is a rapid, non-destructive technique. This study reviews its applications in diverse scientific fields and discusses future prospects.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Context:

  • Fourier-transform infrared (FTIR) microspectroscopy emerged in the mid-1990s.
  • Research is nascent, with limited recognition in China.
  • It offers rapid, non-destructive analysis with microdomain, visualization, high precision, and sensitivity.

Purpose:

  • To summarize the composition, operational principles, and working modes of FTIR microspectroscopy.
  • To investigate the application progress of FTIR microspectroscopy in various scientific domains.
  • To discuss current research challenges and future prospects of FTIR microspectroscopy.

Summary:

  • FTIR microspectroscopy is a powerful analytical technique.
  • Its applications span biomedicine, microbiology, forensic science, materials science, nutrition, feed science, and agricultural products.
  • The review covers fundamental aspects, applications, and future outlook.

Impact:

  • Provides a comprehensive overview of FTIR microspectroscopy for researchers.
  • Highlights the technique's potential across multiple scientific disciplines.
  • Identifies areas for future research and development in FTIR microspectroscopy.