Modified gold surfaces by poly(amidoamine) dendrimers and fructose dehydrogenase for mediated fructose sensing

An electrochemical biosensor for detection of fructose in food samples was developed by immobilization of fructose dehydrogenase (FDH) on cysteamine and poly(amidoamine) dendrimers (PAMAM)-modified gold electrode surface. Electrochemical analysis was carried out by using hexacyanoferrate (HCF) as a mediator and the response time was 35s at +300 mV vs. Ag/AgCl. Moreover, some parameters such as pH, enzyme loading and type of PAMAM (Generations 2, 3 and 4) were investigated. Then, the FDH biosensor was calibrated for fructose in the concentration range of 0.25-5.0mM. To evaluate its utility, the FDH biosensor was applied for fructose analysis in real samples. Finally, obtained data were compared with those measured with HPLC as a reference method.