Related Experiment Video
Updated: May 27, 2026
Partial Heterotopic Hindlimb Transplantation Model in Rats
Published on: June 9, 2021
Vascularized bone marrow transplantation model in rats as an alternative to conventional cellular bone marrow
D Zamfirescu1, C Popovicu, A Stefanescu
1Carol Davila Bucharest Medical University, Bucharest, Romania. dragoszamfirescu@gmail.com
Abstract:
The aim of the study was to follow the development of microchimerism after allogeneic vascularized bone marrow transplantation (VBMT) versus conventional bone marrow transplantation (BMT). In one group, a VBMT model consisted of donor Brown Norway rat hind limb heterotopic transplanted on recipient Lewis rats. An intravenous infusion of donor bone marrow cells in suspension equivalent to that grafted in the vascularized femur limb was administered intravenously to recipient rats in the second group. Cellular microchimerism was investigated in recipients of VBMT versus BMT. Donor-derived cells could be detected in VBMT recipients at 30 and 60 days but not in recipients of intravenous suspension of BMC. VBMT provides a theoretical alternative to conventional cellular bone marrow transplantation by addressing crucial clinical problems such as failure of engraftment or graft-versus-host disease.
