Immunohistochemical evaluation of angiogenesis and tryptase-positive mast cell infiltration in periapical lesions
Sílvio Carlos Albuquerque Lima1, Victor Hugo Toral Rizo, Yara Teresinha Corrêa Silva-Sousa
1School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
Introduction:
Several studies have linked mast cells (MCs) with angiogenesis. The aim of this study was to correlate angiogenesis with MCs in radicular cyst (RC) and periapical granuloma (PG) cases.
Methods:
Forty-eight samples of periapical lesions, diagnosed as RC (n = 24) and PG (n = 24), were included. The microvessel density and microvessel area measured through the immunoexpression of CD105 and CD34 and the MC density measured through the immunoexpression of tryptase were performed.
Results:
MCs were detected in all RCs and PGs (P = .888), mainly in perivascular location and within fibrous stroma. CD34 stained all vessels present in all RC and PG cases. CD105 revealed differential expression, stained preferentially vessels of greater lumen, and showed variable location inside fibrous stroma in both lesions. There was a significant difference of microvessel density determinate by CD34 and CD105 in RCs (r = 0.634, P = .002) and in PGs (r = 0.5709, P = .0036). The difference was also observed when comparing age of the patient in both lesions. Considering the microscopic association between highest concentration of MCs and vascularization, CD105-positive vessels in 50% of RCs and 70.8% of PGs and CD34-positive vessels in 66.7% of RCs and 87.5% of PGs showed areas of close association with MCs.
Conclusions:
These results suggest differential expression of CD105 within RC and PG. There is no difference in angiogenesis and MC density between RC and PG. Moreover, because of the association between MCs with vessels and fibrous stroma, other possible roles of tryptase, in addition to the angiogenic properties, should also be considered.
Insights
Mast cells (MCs) and angiogenesis were evaluated in radicular cysts (RCs) and periapical granulomas (PGs). No significant differences in MC density or angiogenesis were found between RCs and PGs, though MCs showed close association with vessels.
Area of Science:
- Oral pathology
- Immunohistochemistry
- Vascular biology
Background:
- Mast cells (MCs) are implicated in angiogenesis.
- The relationship between MCs and angiogenesis in periapical lesions requires further investigation.
- Radicular cysts (RCs) and periapical granulomas (PGs) are common periapical inflammatory lesions.
Purpose of the Study:
- To correlate angiogenesis with mast cell presence in radicular cysts and periapical granulomas.
- To compare microvessel density and mast cell density between RCs and PGs.
Main Methods:
- Analyzed 48 periapical lesion samples (24 RCs, 24 PGs).
- Assessed microvessel density and area using CD105 and CD34 immunoexpression.
- Quantified mast cell density via tryptase immunoexpression.
Main Results:
- Mast cells were present in all lesions, primarily perivascularly.
- CD34 stained all vessels; CD105 showed differential expression, highlighting larger vessels.
- Significant differences in microvessel density (CD34 and CD105) were observed in RCs and PGs, correlating with patient age.
- Close associations between mast cells and CD105/CD34-positive vessels were noted in a majority of cases.
Conclusions:
- Suggests differential expression of CD105 in RCs and PGs.
- No significant difference in angiogenesis or mast cell density between RCs and PGs.
- Tryptase may have roles beyond angiogenesis due to MC association with vessels and stroma.
