Related Experiment Video
Updated: May 27, 2026
Combination of High Ligation and Intraoperative Embolization Using Polidocanol for Treatment of Varicoceles
Published on: December 22, 2023
Polydeoxyribonucleotide administration improves the intra-testicular vascularization in rat experimental varicocele
Salvatore Arena1, Letteria Minutoli, Francesco Arena
1Department of Urology, University of Messina, Messina, Italy. arenasal@inwind.it
Objective:
To study the effect of PDRN on angiogenesis in a model of varicocele in rats.
Design:
After the creation of experimental varicocele, rats were randomized to one of the four treatments: vehicle, PDRN, DMPX, and PDRN plus DMPX. Twenty-one days after randomization, all animals were euthanized and the left testis was harvested.
Setting:
Academic hospital.
Animal(S):
Male Sprague-Dawley rats were used.
Intervention(S):
A clamp was passed behind the left renal vein distally to the spermatic vein insertion. A silk ligature was placed around the left renal vein at this site and was tied over the top of a probe. The latter was then withdrawn and the vein was allowed to expand. In shams, a suture was placed but it was not tied.
Main Outcome Measure(S):
To assess testicular microvascular density using CD34 immunostaining.
Result(S):
Microvascular density in the varicocele plus PDRN group was significantly higher than in other groups.
Conclusion(S):
PDRN could represent a novel therapeutic strategy for varicocele treatment in subfertile patients, improving the innate pathophysiologic mechanism of neoangiogenesis, through compensatory oxygen and metabolite supply to tubular and extratubular testicular compartments.
