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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: May 27, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

UG repeats/TDP-43 interactions near 5' splice sites exert unpredictable effects on splicing modulation.

Monica Passoni1, Laura De Conti, Marco Baralle

  • 1International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy.

Journal of Molecular Biology
|November 22, 2011
PubMed
Summary

TDP-43 protein

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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Related Experiment Videos

Last Updated: May 27, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Area of Science:

  • Molecular Biology
  • Neuroscience

Background:

  • TDP-43 is a nuclear protein linked to neurodegenerative diseases like ALS and FTD.
  • Cytoplasmic aggregation of TDP-43 in diseased neurons suggests nuclear dysfunction.
  • Understanding TDP-43's nuclear roles is crucial for neurodegeneration research.

Purpose of the Study:

  • Investigate TDP-43's function at 5' splice sites (5'SS).
  • Examine the impact of TDP-43 binding to UG repeats near 5'SS.
  • Determine TDP-43's regulatory role in pre-mRNA splicing.

Main Methods:

  • Utilized artificial and natural splicing substrates.
  • Analyzed TDP-43 binding to UG repeats near exonic 5'SS sequences.
  • Evaluated effects on 5'SS recognition in genes like BRCA1, ETF1, and RXRG.

Main Results:

  • UG repeats near 5'SS are generally weak splicing regulators.
  • TDP-43 binding to UG repeats can activate or suppress 5'SS recognition.
  • Regulatory effects depend on splice site strength and other splicing elements.

Conclusions:

  • TDP-43 binding to UG repeats may influence 5'SS recognition.
  • This role may be critical for mutations weakening 5'SS.
  • The significance of UG repeats near 5'SS requires case-by-case experimental validation.