Related Experiment Video
Updated: May 27, 2026

08:31
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil
Direk Limmathurotsakul1, Vanaporn Wuthiekanun, Premjit Amornchai
1Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. direk@tropmedres.ac
Applied and Environmental Microbiology
|November 22, 2011
Abstract:
Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving.

