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Optimization of a spray drying process to prepare dry powder microparticles containing plasmid nanocomplex
N Mohajel1, A Roholamini Najafabadi, K Azadmanesh
1Aerosol Research Laboratory, Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
International Journal of Pharmaceutics
|November 22, 2011
Summary
This study optimized spray-dried chitosan-plasmid nanocomplexes for lung gene delivery. Statistical analysis identified key factors influencing yield, particle size, and DNA stability for effective delivery systems.
Area of Science:
- Biotechnology
- Pharmaceutical Sciences
- Nanotechnology
Background:
- Effective lung gene delivery requires optimized nanosized nucleic acid systems and microparticle engineering.
- Chitosan-plasmid nanocomplexes are promising for gene delivery but require formulation optimization.
Purpose of the Study:
- To statistically optimize spray-dried formulations of low molecular weight chitosan-plasmid nanocomplexes.
- To identify critical process parameters influencing yield, particle size, DNA stability, and transfection efficiency.
Main Methods:
- Utilized a D-optimal design to optimize spray-dried formulations.
- Investigated the impact of solid content, leucine ratio, inlet temperature, feed rate, and spray air flow rate.
- Applied 2FI and quadratic models to analyze five critical responses.
Main Results:
- Feed rate significantly impacted yield and DNA stability.
- Inlet temperature influenced nanoparticle size, while leucine concentration affected transfection efficiency.
- Two-factor interactions were crucial for microparticle size, nanocomplex size, and DNA stability.
Conclusions:
- Optimized formulations were achieved with maximum tested values for most independent variables, with feed fluid concentration at its midpoint.
- The findings provide a statistically optimized approach for developing effective chitosan-plasmid nanocomplexes for lung gene delivery.
