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Updated: May 27, 2026
Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
Published on: September 4, 2015
Preparation of acute living hippocampal slice from common marmoset (Callithrix jacchus) for synaptic function
Arata Oh-Nishi1, Shigeru Obayashi, Takafumi Minamimoto
1Molecular Neuroimaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan. oh-nishi@nirs.go.jp
Abstract:
We described the preparation of acute living slices from the hippocampus of the neonatal common marmoset (Callithrix jacchus). Slices from a temporal lobe section were prepared quickly using a rotary slicer. By this method, we successfully recorded field potentials, namely, pre-synaptic fiber volley and field excitatory post-synaptic potentials, from the hippocampal CA1 region with conventional electrophysiological techniques, and analyzed the indicators of synaptic function such as input-output curve. This study thus presents an efficient preparation method for acute living hippocampal slice from which synaptic function of the hippocampus in non-human primate can be analyzed.
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