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Updated: May 27, 2026

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine
Chun-Xiao Song1, Tyson A Clark, Xing-Yu Lu
1Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA.
Abstract:
We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with high confidence.

