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Updated: May 27, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Separation of lipid classes by HPLC on a cyanopropyl column
Petter Olsson1, Jan Holmbäck, Bengt Herslöf
1Department of Analytical Chemistry, Stockholm University, Svante Arrhenius väg 16, 10691 Stockholm, Sweden.
Abstract:
A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.
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