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Updated: May 27, 2026
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
Published on: March 3, 2014
Crystallization and preliminary crystallographic studies of the HA3 subcomponent of the type B botulinum neurotoxin
Kohsuke Nishimura1, Kengo Kitadokoro, Yuki Takegahara
1Graduate School of Science and Technology, Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, Japan.
Abstract:
The haemagglutinin subcomponent HA3 of the type B botulinum neurotoxin complex, which is important in toxin absorption from the gastrointestinal tract, has been expressed, purified and subsequently crystallized in two crystal forms at different pH values. Form I belonged to space group R32, with unit-cell parameters a = b = 357.4, c = 249.5 Å, α = β = 90, γ = 120°. Form II belonged to space group I4(1)32, with unit-cell parameters a = b = c = 259.0 Å, α = β = γ = 90°. Diffraction data were collected from these crystals to a resolution of 3.0 Å for both form I and form II.
