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Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from
Cecilia Ortíz1, Nicole Larrieux, Andrea Medeiros
1Redox Biology of Trypanosomes Laboratory, Institut Pasteur de Montevideo, Mataojo 2020, 11400 Montevideo, Uruguay.
Abstract:
An N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues was crystallized both in its apo form and in a binary complex with glucose 6-phosphate. The crystals both belonged to space group P2(1) and diffracted to 2.85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure.
