Related Experiment Video
Updated: May 27, 2026

Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
STAT5 in human basophils: IL-3 is required for its FcεRI-mediated phosphorylation
Marjoke M Verweij1, Vito Sabato, Sara Nullens
1Department of Immunology-Allergology-Rheumatology, Faculty of Medicine, University of Antwerp, Antwerpen, Belgium.
Abstract:
Basophils are key effector cells in allergic inflammatory reactions. However, the mechanisms of FcεRI-induced degranulation are complex and remain to be disentangled. Signal transducer and activator of transcription (STAT) molecules modulate various cell functions. STAT5 appears to be essential for IgE-mediated mast cell function, but its role in human basophils after cross-linking FcεRI is unknown. In this study, STAT5 phosphorylation was investigated by flow cytometry, and combined with analyses of the degranulation marker CD63 at single cell level. Kinetics of STAT5 phosphorylation were studied in basophils of birch pollen allergic patients and showed a fast phosphorylation induced by interleukin (IL)-3, but not with antigen alone. Stimulating basophils with a mixture of allergen and IL-3 resulted in a two to three fold higher phosphorylation of STAT5 than induced by IL-3 alone. In the presence of IL-3, antigen elicited a dose-dependent STAT5 response. In conclusion, this study demonstrates that STAT5 in human basophils is activated through both the IL-3 and the FcεRI signaling pathway.
Related Concept Videos
The JAK-STAT Signaling Pathway
TGF - β Signaling Pathway
Intracellular Signaling Affects Focal Adhesions
Some...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Mechanism of Filopodia Formation
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Differentiation of Common Myeloid Progenitor Cells

