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Updated: May 27, 2026

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

Published on: May 30, 2011

Robust method for 3D arterial spin labeling in mice.

Brige Paul Chugh¹, Jonathan Bishop, Yu-Qing Zhou

¹Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada. brige@phenogenomics.ca

Magnetic Resonance in Medicine

|November 22, 2011

Summary

This study presents a new method for accurately mapping cerebral blood flow (CBF) in mice using arterial spin labeling. The technique overcomes key challenges, enabling precise measurement of blood flow across brain regions.

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Area of Science:

Neuroimaging
Physiology
Medical Physics

Background:

Arterial spin labeling (ASL) offers potential for accurate cerebral blood flow (CBF) quantification in mouse models.
Challenges in mouse CBF mapping include limited physiological data, stringent MRI gradient requirements, and magnetization transfer (MT) artifacts.

Purpose of the Study:

To develop and validate a robust 3D CBF mapping technique for mouse brains using amplitude-modulated continuous ASL.
To address challenges in physiological data acquisition, gradient performance, and MT artifacts for improved CBF accuracy.

Main Methods:

Characterized carotid artery blood velocity using Doppler ultrasound for CBF modeling.
Optimized inversion efficiency via simulations under MRI gradient duty cycle constraints.

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Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

Published on: November 30, 2011

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Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

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Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

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Implemented a rapid slice positioning algorithm and a binary spin bath model to correct for MT effects.

Main Results:

Achieved reproducible inversion efficiency (0.67 ± 0.03).
Demonstrated statistically significant regional CBF variations across 12 brain areas (P < 0.0001).
Reported a mean whole-brain CBF of 219 ± 6 mL/100 g/min in 10 mice.

Conclusions:

The developed AM continuous ASL method provides robust and reproducible 3D CBF mapping in mice.
The study successfully addressed key technical challenges, improving the accuracy of perfusion quantification in small animal models.
Findings highlight significant regional heterogeneity in mouse brain CBF, relevant for preclinical research.