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Related Concept Videos

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

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Updated: May 27, 2026

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

YeTFaSCo: a database of evaluated yeast transcription factor sequence specificities.

Carl G de Boer1, Timothy R Hughes

  • 1Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada.

Nucleic Acids Research
|November 22, 2011
PubMed
Summary

A new database, YeTFaSCo, compiles yeast transcription factor (TF) binding specificities. It offers a comprehensive and quality-evaluated collection to improve TF network analysis.

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Last Updated: May 27, 2026

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Published on: February 11, 2019

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Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Saccharomyces cerevisiae is a key model organism for studying transcriptional networks.
  • Numerous DNA-binding sequence specificities (motifs) exist for yeast transcription factors (TFs), but inconsistencies between studies complicate analysis.

Purpose of the Study:

  • To create a comprehensive and quality-evaluated compendium of yeast TF binding specificities.
  • To provide tools for motif analysis and improve the reliability of TF network studies.

Main Methods:

  • Compilation of 1709 motifs for 256 yeast TFs.
  • Evaluation of motifs using quality metrics such as correlation with ChIP-chip data, gene expression, GO terms, and inter-study agreement.
  • Development of the YeTFaSCo database and associated web tools.

Main Results:

  • The YeTFaSCo database offers a more comprehensive collection of yeast TF specificities than existing resources.
  • Motifs are evaluated using objective quality metrics, including an index of 'expert-curated' motifs with confidence assessments.
  • The database includes tools for sequence scanning, genome-browser tracks, and similarity searches.

Conclusions:

  • YeTFaSCo provides a valuable, quality-controlled resource for yeast TF binding motifs.
  • The compendium and tools aim to resolve inconsistencies and facilitate more accurate transcriptional network analyses in Saccharomyces cerevisiae.