Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Reaction Mechanisms: Rate-limiting Step Approximation01:29

Reaction Mechanisms: Rate-limiting Step Approximation

The rate-determining step, or RDS, in a chemical reaction is the slowest step that determines the overall reaction rate. It is identified by using the observed rate law and typically involves approximation methods like the RDS approximation or the steady-state approximation.In the RDS approximation, also known as the rate-limiting-step or equilibrium approximation, the reaction mechanism consists of one or more reversible reactions near equilibrium, followed by a slower RDS, and then one or...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
SN1 Reaction: Kinetics02:05

SN1 Reaction: Kinetics

In an SN2 reaction, the reaction rate depends on both the type of nucleophile and the substrate. A hindered tertiary alkyl halide is practically inert to the SN2 mechanism despite using a strong nucleophile.
However, Sir Christopher Ingold and Edward D. Hughes, who studied the kinetics of various nucleophilic substitution reactions, noticed that a tertiary alkyl halide does undergo a nucleophilic substitution reaction in the presence of a weak nucleophile. While studying the substitution...
Measuring Reaction Rates03:09

Measuring Reaction Rates

Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical field in...
SN2 Reaction: Kinetics02:14

SN2 Reaction: Kinetics

Kinetic Studies and Significance
In a chemical reaction, a relationship exists between the concentration of reactants and the rate at which the reaction proceeds. The study to measure this relationship is known as the kinetics of a chemical reaction. Kinetic studies are used to deduce the rate law of a chemical reaction, which provides information about the species involved during the transition state of the rate-determining step. Thus, kinetic studies help to derive the mechanism of a reaction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic Testing in Gastrointestinal Polyposis Syndromes: Considerations in Pediatrics.

Genes·2026
Same author

Artificial intelligence in pediatric endoscopy for hereditary polyposis syndromes: promises and challenges.

Current opinion in pediatrics·2026
Same author

Incidental Colorectal Adenomas in Adolescents: Clinical Management, Genetic Evaluation, and Surveillance.

Journal of gastroenterology and hepatology·2026
Same author

Pediatric Lynch syndrome: Clinical, genotypic, and left-sided patterns of colorectal cancer.

Journal of pediatric gastroenterology and nutrition·2026
Same author

Advances in pediatric video capsule endoscopy: current applications and future directions.

Frontiers in pediatrics·2026
Same author

The utility of artificial intelligence in visualization of pediatric gastrointestinal mucosa.

Frontiers in pediatrics·2026

Related Experiment Video

Updated: May 27, 2026

A Web Tool for Generating High Quality Machine-readable Biological Pathways
08:01

A Web Tool for Generating High Quality Machine-readable Biological Pathways

Published on: February 8, 2017

SABIO-RK--database for biochemical reaction kinetics.

Ulrike Wittig1, Renate Kania, Martin Golebiewski

  • 1Scientific Databases and Visualization Group, Heidelberg Institute for Theoretical Studies, gGmbH, Schloss-Wolfsbrunnenweg 35, 69118 Heidelberg, Germany. Ulrike.Wittig@h-its.org

Nucleic Acids Research
|November 22, 2011
PubMed
Summary

SABIO-RK is a manually curated database for biochemical reaction kinetics. It provides standardized kinetic parameters, rate laws, and experimental conditions for broad biological research and modeling.

More Related Videos

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Related Experiment Videos

Last Updated: May 27, 2026

A Web Tool for Generating High Quality Machine-readable Biological Pathways
08:01

A Web Tool for Generating High Quality Machine-readable Biological Pathways

Published on: February 8, 2017

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Area of Science:

  • Biochemistry
  • Systems Biology
  • Bioinformatics

Background:

  • Accurate kinetic data is crucial for understanding cellular processes and building predictive biological models.
  • Existing resources may lack comprehensive, standardized, and manually curated kinetic information.
  • Integrating diverse kinetic data is essential for advancing systems biology.

Purpose of the Study:

  • To present SABIO-RK, a comprehensive, web-accessible database of biochemical reaction kinetics.
  • To highlight the manual curation and annotation process ensuring data quality and consistency.
  • To provide flexible access via web interfaces and web services, including SBML export for modeling.

Main Methods:

  • Manual extraction and curation of kinetic data from scientific literature.
  • Inclusion of data from direct laboratory submissions.
  • Development of web-based user interfaces and programmatic web services for data access.
  • Annotation of kinetic parameters with reaction, biological source, and experimental conditions.
  • Automated consistency checks to ensure data integrity.

Main Results:

  • SABIO-RK contains a broad range of kinetic parameters for biochemical reactions across various organisms.
  • Data includes kinetic rate laws, equations, and detailed experimental conditions.
  • Standardized annotations facilitate data interpretation and cross-referencing.
  • Web services enable seamless integration with other bioinformatics tools and modeling software.

Conclusions:

  • SABIO-RK serves as a valuable, high-quality resource for researchers in biochemistry and systems biology.
  • The database supports the development and validation of kinetic models.
  • Standardized and accessible kinetic data accelerates biological discovery and understanding.