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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: May 27, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Modern analytical techniques in metabolomics analysis.

Aihua Zhang1, Hui Sun, Ping Wang

  • 1National TCM Key Lab of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.

The Analyst
|November 22, 2011
PubMed
Summary
This summary is machine-generated.

Metabolomics analyzes small molecules for disease insights. Combining analytical techniques like GC, HPLC, and MS enhances metabolite detection and coverage for systems biology.

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

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Last Updated: May 27, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Systems Biology

Background:

  • Metabolomics involves comprehensive assessment and quantification of endogenous metabolites.
  • Small-molecule metabolites are crucial for understanding biological systems and disease phenotypes.
  • Metabolites encompass diverse structures like lipids, amino acids, and carbohydrates, posing analytical challenges.

Purpose of the Study:

  • To review the application, strengths, and limitations of hyphenated analytical techniques in metabolomics.
  • To highlight the role of integrated analytical tools in enhancing metabolite detection and coverage.
  • To discuss the impact of advanced analytical platforms on the integration of metabolomics into systems biology.

Main Methods:

  • Utilized a range of analytical platforms including Gas Chromatography (GC), High-Performance Liquid Chromatography (HPLC), Ultra-Performance Liquid Chromatography (UPLC), Capillary Electrophoresis (CE) coupled to Mass Spectrometry (MS), and Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Discussed the combined use of modern instrumental analytical approaches for comprehensive metabolome analysis.
  • Reviewed illustrative examples of hyphenated techniques and integrated tools in metabolomic research.

Main Results:

  • No single analytical platform can detect all metabolites due to the metabolome's complexity and metabolite diversity.
  • Integrated analytical platforms provide sensitive and reliable detection of thousands of metabolites in biological samples.
  • Combined techniques significantly increase the coverage of detected metabolites compared to single-analysis methods.

Conclusions:

  • The combined use of modern analytical platforms is essential for comprehensive metabolomic analysis.
  • Integrated tools are beneficial for increasing metabolite detection coverage, which is unachievable with single techniques.
  • Continued development of analytical platforms will accelerate the widespread use and integration of metabolomics into systems biology.