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Updated: May 27, 2026
CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Study of DNA-deltamethrin binding by voltammetry, competitive fluorescence, thermal denaturation, circular dichroism,
Farhad Ahmadi1, Nasibeh Jamali
1Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Islamic Republic of Iran. fahmadi@kums.ac.ir
Abstract:
The interaction of deltamethrin (DM), a synthetic insecticide, with calf thymus DNA was studied. The cyclic voltammetric (CV) results revealed that DM has two irreversible cathodic peaks. The first peak (a) was devoted to reduction of -CN by 4 electrons and the second peak (b) was devoted to reduction of the -C = C- moiety by two electrons. By using non-linear regression analysis of CV data of peak (a), the binding constant, binding site size, and diffusion coefficient for free DM (D(f)) and DNA-DM (D(b)) were calculated as: 2.6 × 10(4), 1.6, 3.2 × 10(-4)Cm(2) S(-1), and 8.5 × 10(-6)Cm(2) S(-1), respectively. The thermal denaturation, competitive fluorescence, and AFM results revealed that the mode of interaction may be non-intercalative. Also the circular dichroism spectra showed that the conformation of CT DNA was converted from right-handed B-DNA to A-DNA due to the destacking of the adjacent guanine bases in pH 7.3 solution.
