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Related Experiment Video

Updated: May 27, 2026

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Published on: September 11, 2019

The CD4C/HIV(Nef)transgenic model of AIDS.

Paul Jolicoeur¹

¹Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada. paul.jolicoeur@ircm.qc.ca

Current HIV Research

|November 23, 2011

Summary

Transgenic mice expressing HIV-1 Nef in CD4+ cells develop AIDS-like symptoms. This model aids in studying human immunodeficiency virus (HIV) pathogenesis and potential therapies.

Area of Science:

Immunology
Virology
Genetics

Background:

Human immunodeficiency virus (HIV) infection leads to Acquired Immunodeficiency Syndrome (AIDS).
The HIV-1 Nef protein plays a critical role in viral pathogenesis and disease progression.
Understanding HIV-1 Nef's function in vivo is crucial for developing effective treatments.

Purpose of the Study:

To develop and characterize a transgenic mouse model expressing HIV-1 Nef.
To investigate the effects of HIV-1 Nef expression in relevant target cells.
To establish a model for studying AIDS pathogenesis and evaluating therapeutic strategies.

Main Methods:

Generation of CD4C/HIVNef transgenic (Tg) mice.
Expression of HIV-1 Nef driven by the human CD4 gene regulatory sequences.

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Assessment of disease development and characteristics in Tg mice.

Main Results:

CD4C/HIVNef Tg mice exhibit a severe disease phenotype.
The observed disease closely mimics key characteristics of human AIDS.
This model provides a platform for studying HIV-1-induced immunodeficiency.

Conclusions:

CD4C/HIVNef Tg mice serve as a valuable model for AIDS research.
The model facilitates the study of HIV-1 Nef's role in disease.
Further research using this model can advance understanding of HIV pathogenesis and treatment.