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A cDNA encoding human ribosomal protein S24
S J Brown1, A Jewell, C G Maki
1Center for Basic Cancer Research, Kansas State University, Manhattan 66506.
Gene
|July 16, 1990
Summary
Researchers isolated and sequenced a human gene for 40S ribosomal protein S24. This human protein is nearly identical to a previously identified Xenopus laevis protein, suggesting conserved function in ribosomal structure.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Ribosomal proteins are essential components of ribosomes, the cellular machinery responsible for protein synthesis.
- The human 40S ribosomal subunit plays a critical role in translation initiation.
Purpose of the Study:
- To isolate and determine the nucleotide sequence of the human cDNA encoding the 40S ribosomal protein S24.
- To compare the human S24 sequence with known ribosomal proteins from other species.
Main Methods:
- Complementary DNA (cDNA) isolation techniques.
- Nucleotide sequencing of the isolated cDNA.
- Bioinformatic analysis for sequence comparison.
Main Results:
- Successfully isolated and sequenced the human cDNA for 40S ribosomal protein S24.
- The deduced amino acid sequence of human S24 shows high similarity to a Xenopus laevis r-protein previously identified as S19.
- Molecular weight of human S24 is 15,425 Da.
Conclusions:
- The human 40S ribosomal protein S24 sequence has been elucidated.
- The high degree of sequence identity suggests evolutionary conservation of this ribosomal protein between humans and Xenopus laevis.
- This finding contributes to understanding the structural and functional conservation of ribosomal proteins across species.