Propagation characteristics of photomechanical waves and their application to gene delivery into deep tissue

Targeted gene transfection can be achieved by the use of photomechanical waves (PMWs) generated by irradiating a solid material with high-power nanosecond laser pulses. To examine the treatable tissue depth, we investigated propagation characteristics of PMWs and depth-dependent properties of gene transfection with different laser fluences and spot diameters. Pressure characteristics of PMWs were measured at different propagation distances using tissue phantoms and their propagation was imaged by shadowgraphing. Phantoms with various thicknesses were placed on rat dorsal skin that had been injected with plasmid DNA coding for a reporter gene and three pulses of PMWs were applied from the top of each phantom. Significant gene expression was observed in the skin even under a 15-mm-thick tissue phantom and the depth-dependent relationships between PMW parameters and gene expression level were revealed. The data obtained will be useful for determining appropriate laser parameters for PMW-based gene transfer into deep-located tissue.