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Enzyme synthesis of L-tryptophan
R Zeman1, J Plachý, H Bulantová
1Research Institute of Antibiotics and Biotransformations, Roztoky near Prague, Czechoslovakia.
Folia Microbiologica
|January 1, 1990
Abstract:
Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Production of 32.4 g/L of L-tryptophan was reached after 48 h under optimal conditions.