Agreement between retinal nerve fiber layer measures from Spectralis and Cirrus spectral domain OCT

An assessment of the retinal nerve fiber layer (RNFL) provides important information on the health of the optic nerve. There are several non-invasive technologies, including spectral domain optical coherence tomography (SD OCT), that can be used for in vivo imaging and quantification of the RNFL, but often there is disagreement in RNFL thickness between clinical instruments. The purpose of this study was to investigate the influence of scan centration, ocular magnification, and segmentation on the degree of agreement of RNFL thickness measures by two SD OCT instruments.

RNFL scans were acquired from 45 normal eyes using two commercially available SD OCT systems. Agreement between RNFL thickness measures was determined using each instrument's algorithm for segmentation and a custom algorithm for segmentation. The custom algorithm included ocular biometry measures to compute the transverse scaling for each eye. Major retinal vessels were identified and removed from RNFL measures in 1:1 scaled images. Transverse scaling was also used to compute the RNFL area for each scan.

Instrument-derived global RNFL thickness measured from the two instruments correlated well (R(2) = 0.70, p < 0.01) but with significant differences between instruments (mean of 6.7 microm; 95% limits of agreement of 16.0 microm to -2.5 microm, intraclass correlation coefficient = 0.62). For recentered scans with custom RNFL segmentation, the mean difference was reduced to 0.1 microm (95% limits of agreement 6.1 to -5.8 microm, intraclass correlation coefficient = 0.92). Global RNFL thickness was related to axial length (R = 0.24, p < 0.01), whereas global RNFL area measures were not (R(2) = 0.004, p = 0.66). Major retinal vasculature accounted for 11.3 ± 1.6% (Cirrus) or 11.8 ± 1.4% (Spectralis) of the RNFL thickness/area measures.

Sources of disagreement in RNFL measures between SD-OCT instruments can be attributed to the location of the scan path and differences in their retinal layer segmentation algorithms. In normal eyes, the major retinal vasculature accounts for a significant percentage of the RNFL and is similar between instruments. With incorporation of an individual's ocular biometry, RNFL area measures are independent of axial length, with either instrument.