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A novel exonuclease III aided amplification method for sensitive nucleic acid detection based on single walled carbon

Hui Chen1, Jiaqin Wang, Guohai Liang

  • 1Department of Chemistry, CAM & IBS, Fudan University, Shanghai, PR China.

Chemical Communications (Cambridge, England)
|November 23, 2011
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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