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Related Experiment Video

Updated: May 27, 2026

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

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Published on: November 10, 2017

Cell cycle-specific function of Ikaros in human leukemia.

Zhanjun Li¹, Chunhua Song, Hongsheng Ouyang

¹Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.

Pediatric Blood & Cancer

|November 23, 2011

Summary

Human Ikaros protein function is cell cycle-specific, regulated by CK2 phosphorylation in acute lymphoblastic leukemia (ALL). This discovery highlights unique human Ikaros functions and identifies the CK2 pathway as a potential therapeutic target for ALL.

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Last Updated: May 27, 2026

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Published on: May 14, 2016

Area of Science:

Molecular Biology
Cell Biology
Cancer Research

Background:

Ikaros loss is linked to B and T cell leukemia development.
Human Ikaros function and regulation, particularly during the cell cycle, remain poorly understood.
Previous research primarily utilized murine models, with limited data on human Ikaros.

Purpose of the Study:

To investigate the function and regulation of human Ikaros isoforms during the cell cycle in human acute lymphoblastic leukemia (ALL).
To elucidate the mechanisms controlling human Ikaros activity throughout the cell cycle.

Main Methods:

Electromobility shift assay (EMSA) to assess DNA-binding activity.
Confocal microscopy for subcellular localization studies.
Phosphopeptide mapping to identify phosphorylation sites.

Main Results:

Human Ikaros DNA-binding activity dynamically changes during the cell cycle.
In S phase, Ikaros DNA-binding to target genes decreases while binding to pericentromeric heterochromatin is maintained.
CK2 kinase-mediated phosphorylation controls these S phase-specific alterations in Ikaros function.
Human Ikaros isoform localization differs from murine models, suggesting unique human functions.

Conclusions:

Human Ikaros function is cell cycle-dependent and regulated by CK2 phosphorylation during S phase in T-cell and B-cell ALL.
Observed differences between murine and human Ikaros underscore the necessity of human cell studies in ALL research.
The CK2 pathway is identified as a promising therapeutic target for ALL treatment.