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Multilocus variable-number tandem-repeat analysis for subtyping Salmonella enterica serovar Gallinarum
Min S Kang1, Yong K Kwon, Jae Y Oh
1National Veterinary Research & Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi, Republic of Korea.kangmskr@korea.kr
Avian Pathology : Journal of the W.V.P.A
|November 24, 2011
Summary
A new Multilocus variable-number tandem-repeat analysis (MLVA) assay effectively subtypes Salmonella enterica serovar Gallinarum, the cause of fowl typhoid. This method offers higher discrimination than traditional PFGE for epidemiological studies.
Area of Science:
- Veterinary Microbiology
- Molecular Epidemiology
- Bacterial Genotyping
Background:
- Fowl typhoid, caused by Salmonella enterica serovar Gallinarum, is a significant global poultry disease.
- Multilocus variable-number tandem-repeat analysis (MLVA) is a valuable tool for subtyping Salmonella serovars.
Purpose of the Study:
- To develop and evaluate a simple MLVA assay for Salmonella enterica serovar Gallinarum.
- To compare the discriminatory power of the MLVA assay with pulsed-field gel electrophoresis (PFGE).
Main Methods:
- Genome analysis to identify potential VNTR loci.
- Development of multiplex PCR assays for selected VNTR markers.
- In vitro stability assessment of VNTR markers.
- MLVA and PFGE analysis of 68 Salmonella enterica serovar Gallinarum strains.
Main Results:
- A four-locus MLVA assay was developed and validated.
- MLVA distinguished 26 allelic profiles with a Simpson's diversity index of 0.918.
- MLVA demonstrated higher discriminatory power than PFGE (23 patterns, 0.874 diversity index) and resolved strains with identical PFGE patterns.
Conclusions:
- The developed MLVA assay is a highly discriminatory genotyping method for Salmonella enterica serovar Gallinarum.
- MLVA serves as a valuable adjunct to routine PFGE analysis for molecular epidemiological investigations of fowl typhoid.
