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A microplate method for the determination of amino groups in monoclonal antibodies
Hybridoma
|August 1, 1990
Abstract:
The total number of amino groups in monoclonal antibodies can be estimated via a colorimetric assay using trinitrobenzene sulfonic acid (A.F.S.A. Habeeb (1966) Anal. Biochem. 14, 328-336). This work describes the adaptation of this method to a microtiter plate in order to rapidly and reliably assay large numbers of samples.