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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: May 27, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Refractometric sensing with fluorescent-core microcapillaries.

C P K Manchee1, V Zamora, J W Silverstone

  • 1Department of Physics, University of Alberta, Edmonton, AB, Canada.

Optics Express
|November 24, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel capillary-based microfluidic sensor using fluorescent silicon quantum dots to detect refractive index changes. The whispering gallery mode sensor achieved a sensitivity of 9.8 nm/RIU, with potential for higher performance.

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Area of Science:

  • Optics and Photonics
  • Materials Science
  • Microfluidics

Background:

  • Capillary structures offer potential for microfluidic refractive index sensing.
  • Whispering gallery mode (WGM) resonances are sensitive to changes in the surrounding medium's refractive index.

Purpose of the Study:

  • To demonstrate a capillary-type fluorescent core microcavity sensor.
  • To investigate the use of silicon quantum dots (QDs) for WGM resonance enhancement in microfluidic applications.

Main Methods:

  • A microcapillary coated with fluorescent silicon QDs was fabricated.
  • Sucrose solutions were flowed through the capillary, and fluorescence WGM spectra were measured.
  • Optical modeling was used to analyze the electric field confinement and resonance behavior.

Main Results:

  • The sensor exhibited WGM resonances in its fluorescence spectrum due to the QD layer.
  • A refractometric sensitivity of 9.8 nm/RIU (up to 13.8 nm/RIU) was achieved.
  • A maximum detection limit of approximately 7.2 x 10(-3) RIU was determined.

Conclusions:

  • The developed capillary microcavity sensor effectively utilizes WGM resonances for refractive index sensing.
  • Optimization of QD film thickness could significantly enhance sensor sensitivity and detection limits, potentially reaching 100 nm/RIU and 10(-5) RIU, respectively.