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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 27, 2026

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
11:56

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Autofocusing method using fluorescence detection for precise two-photon nanofabrication.

Byung Je Jung1, Hong Jin Kong, Byoung Goo Jeon

  • 1Department of physics, KAIST, 373-1 Guseong-dong, Yuseong-gu, Deajeon 305-701, Korea. blake-j@kaist.ac.kr

Optics Express
|November 24, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces an automated laser focusing method for two-photon nanofabrication using resin fluorescence. The technique precisely locates the focal point, enabling high-resolution 3D structure fabrication without defects.

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Fabrication of 1-D Photonic Crystal Cavity on a Nanofiber Using Femtosecond Laser-induced Ablation
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Area of Science:

  • Materials Science
  • Optical Engineering
  • Nanotechnology

Background:

  • Two-photon fabrication requires precise laser focusing for high-resolution 3D micro/nanostructure fabrication.
  • Current focusing methods can be time-consuming and may leave unwanted marks.

Purpose of the Study:

  • To develop an automated, fluorescence-guided autofocusing method for two-photon nanofabrication.
  • To improve the accuracy and efficiency of laser beam focusing in resin-based 3D printing.

Main Methods:

  • Utilizing visible fluorescence emission generated by two-photon absorption (TPA) in the resin.
  • Employing a CCD camera to capture fluorescence images and analyzing pixel counts against focus position.
  • Identifying the optimal focus by detecting an abrupt change in fluorescence signal in the pre-TPA region.

Main Results:

  • The autofocusing method achieved focusing errors within -100 nm to +200 nm.
  • The process demonstrated no residual polymerized marks on the substrate.
  • Successful fabrication of a 20-layer pyramid structure without layer loss verified the method's utility.

Conclusions:

  • The proposed fluorescence-detection autofocusing method is accurate and reliable for two-photon nanofabrication.
  • This technique offers a non-destructive and efficient approach to achieve precise laser focusing.
  • The method enables high-fidelity fabrication of complex 3D nanostructures.