Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Screening anxiety via contrastive autobiographical recall.

Frontiers in digital health·2026

Same author

Differentiating malignancy from liver parenchyma in Ex-Vivo OCT images using anomaly detection.

Scientific reports·2026

Same author

Classification of Pancreatic Cancer and Normal Tissue in 2D and 3D Optical Coherence Tomography Images Using Convolutional Neural Networks: A Comparative Study.

Cancers·2026

Same author

Towards FAIR and federated data ecosystems for interdisciplinary research.

PLoS computational biology·2026

Same author

Tephra-mediated manganese cycling shapes coral responses to coastal sedimentation.

Scientific reports·2026

Same author

PARANOiD: Pipeline for Automated Read ANalysis of iCLIP Data.

Bioinformatics (Oxford, England)·2025

Search research articles

Related Experiment Video

Updated: May 27, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Fast and low-cost structured light pattern sequence projection.

Patrick Wissmann¹, Frank Forster, Robert Schmitt

¹Laboratory for Machine Tools and Production Engineering (WZL), University of Technology Aachen (RWTH Aachen), Manfred-Weck Building, Steinbachstrasse 19, 52074 Aachen, Germany. patrick.wissmann@siemens.com

|November 24, 2011

Summary

This summary is machine-generated.

This study introduces a fast, low-cost structured light projection method using a rotating spatial light modulator. It achieves high-speed 3D measurements with precise detail, enabling kHz projection frequencies and 20 Hz reconstruction rates.

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Related Experiment Videos

Last Updated: May 27, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Area of Science:

Optics and Photonics
3D Metrology
Computer Vision

Background:

Structured light projection is crucial for 3D measurement.
Existing methods face limitations in speed, cost, and resolution.
High-speed, high-accuracy 3D reconstruction remains a challenge.

Purpose of the Study:

To develop a high-speed, low-cost structured light projection system.
To enable kHz projection frequencies and micrometer-level pattern resolution.
To achieve accurate 3D measurements at high frame rates.

Main Methods:

Utilized a fast rotating binary spatial light modulator for pattern projection.
Developed a ray-cone triangulation method based on circular fringe patterns.
Integrated the projection system with coded phase shift (CPS) pattern sequences.
Performed detailed optical calibration for precise measurements.

Main Results:

Achieved projection frequencies potentially in the kHz domain.
Enabled pattern rasterization with a 2 μm pixel size.
Demonstrated 12-bit grayscale linearity.
Successfully performed high-accuracy 3D measurement at 200 Hz projection frequency.
Attained a 3D reconstruction rate of 20 Hz.

Conclusions:

The proposed method offers a high-speed and cost-effective solution for structured light projection.
Ray-cone triangulation effectively handles the circular fringe patterns for accurate 3D reconstruction.
The system demonstrates significant potential for applications requiring rapid and precise 3D measurements.