Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cytomegalovirus Disease

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...

Human Virome

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluating vectors for the design of a spillover-disrupting Lassa virus transmissible vaccine.

PLoS computational biology·2026

Same author

Targeting RUNX1 protects against diastolic dysfunction in a two-hit mouse model of heart failure with preserved ejection fraction.

Cardiovascular research·2026

Same author

Virion proteomics of genetically intact HCMV reveals a regulator of envelope glycoprotein composition that protects against humoral immunity.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

The contribution of other effective area-based conservation measures (OECMs) to protecting global biodiversity.

Nature communications·2025

Same author

A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples.

Parasite (Paris, France)·2025

Same author

Inherited chromosomally integrated human herpesvirus 6: regional variation in prevalence, association with angina, and identification of ancestral viral lineages in two large UK studies.

Journal of virology·2025

Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 27, 2026

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

High-resolution human cytomegalovirus transcriptome.

Derek Gatherer¹, Sepehr Seirafian, Charles Cunningham

¹Medical Research Council-University of Glasgow Centre for Virus Research, Glasgow G11 5JR, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America

|November 24, 2011

Summary

This summary is machine-generated.

Deep sequencing reveals human cytomegalovirus (HCMV) transcription complexity, with most RNA producing noncoding transcripts and frequent alternative splicing affecting protein-coding genes. This highlights sophisticated viral functionality.

More Related Videos

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m⁶A and m⁵C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

Related Experiment Videos

Last Updated: May 27, 2026

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m⁶A and m⁵C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

Area of Science:

Virology
Genomics
Molecular Biology

Background:

Human cytomegalovirus (HCMV) is a significant pathogen with a complex lifecycle.
Understanding HCMV gene expression is crucial for developing antiviral strategies.

Purpose of the Study:

To comprehensively map the HCMV transcriptome during infectious virion production.
To identify novel noncoding RNAs, splice variants, and protein-coding regions.

Main Methods:

Deep sequencing of polyadenylated viral RNA.
Confirmation of findings using RT-PCR and RACE (Rapid Amplification of cDNA Ends).

Main Results:

The majority of HCMV RNA (65.1%) consists of four major noncoding transcripts.
Extensive alternative splicing affects 58 protein-coding genes, with frequent splicing in four specific genes.
Four previously unrecognized protein-coding regions (RL8A, RL9A, UL150A, US33A) were annotated, with UL150A protein expression confirmed.

Conclusions:

HCMV transcription is highly complex and multifaceted, suggesting sophisticated viral functions.
Deep sequencing is a powerful tool for elucidating the genomics of nuclear DNA viruses like HCMV.