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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Organisms00:53

Transgenic Organisms

Overview
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...

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Related Experiment Video

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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

pTransgenesis: a cross-species, modular transgenesis resource.

Nick R Love1, Raphael Thuret, Yaoyao Chen

  • 1Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK.

Development (Cambridge, England)
|November 24, 2011
PubMed
Summary

Researchers developed pTransgenesis, a modular cloning system to streamline DNA construct creation for transgenesis experiments. This system facilitates cross-species collaborations by standardizing essential elements for creating transgenic organisms efficiently.

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Efficient transgenesis is crucial for studying conserved biological properties across species.
  • Current DNA construction methods for transgenesis can be time-consuming, hindering research and collaboration.

Purpose of the Study:

  • To develop a modular, recombination-based cloning system to expedite the creation of transgenic organisms.
  • To facilitate cross-species collaboration by standardizing transgenesis elements.

Main Methods:

  • Developed the pTransgenesis modular cloning system incorporating four key transgenesis elements.
  • Created a library of coding sequences (fluorescent proteins, Gal4, Cre-recombinase) compatible with modular elements.
  • Validated the system in Xenopus for Gal4-UAS expression, Cre-loxP fate-mapping, and generating transgenic lines.

Main Results:

  • Demonstrated successful Gal4-UAS binary expression and Cre-loxP-mediated fate-mapping in Xenopus.
  • Established novel, tissue-specific transgenic lines using the pTransgenesis system.
  • Confirmed compatibility of pTransgenesis with transgenesis in Drosophila, zebrafish, and mammalian cell models.

Conclusions:

  • The pTransgenesis resource streamlines DNA construct creation for transgenesis.
  • It fosters cross-model standardization of commonly used transgenesis elements.
  • Facilitates collaboration among researchers using diverse model organisms.