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Updated: May 27, 2026
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Cestode antigens induce a tolerogenic-like phenotype and inhibit LPS inflammatory responses in human dendritic cells
César A Terrazas1, Fausto Sánchez-Muñoz, Ana M Mejía-Domínguez
1Biomedicine Unit, Facultad de Estudios Superiores Iztacala, UNAM, México City, México.
Abstract:
Pathogens have developed strategies to modify Dendritic Cells (DCs) phenotypes and impair their functions in order to create a safer environment for their survival. DCs responses to helminths and their derivatives vary among different studies. Here we show that excretory/secretory products of the cestode Taenia crassiceps (TcES) do not induce the maturation of human DCs judged by a lack of increment in the expression of CD83, HLA-DR, CD80 and CD86 molecules but enhanced the production of IL-10 and positively modulated the expression of the C-type lectin receptor MGL and negatively modulated the expression of DC-SIGN. Additionally, these antigens were capable of down-modulating the inflammatory response induced by LPS in these cells by reducing the expression of the maturation markers and the production of the inflammatory cytokines IL-1β, TNF, IL-12 and IL-6. The effects of TcES upon the DCs responses to LPS were stronger if cells were exposed during their differentiation to the helminth antigens. All together, these findings suggest the ability of TcES to induce the differentiation of human DCs into a tolerogenic-like phenotype and to inhibit the effects of inflammatory stimuli.
Insights
Excretory/secretory products from Taenia crassiceps (TcES) induce human dendritic cells (DCs) to adopt a tolerogenic phenotype. TcES inhibits inflammatory responses, suggesting a role in immune modulation.
Area of Science:
- Immunology
- Parasitology
- Cell Biology
Background:
- Pathogens often manipulate host dendritic cells (DCs) to evade immune responses.
- DC responses to helminths are complex and require further elucidation.
- Taenia crassiceps excretory/secretory products (TcES) are potential immunomodulators.
Purpose of the Study:
- To investigate the effect of TcES on human dendritic cell (DC) maturation and function.
- To determine if TcES can modulate inflammatory responses induced by lipopolysaccharide (LPS).
- To explore the potential of TcES in inducing a tolerogenic DC phenotype.
Main Methods:
- Human DCs were cultured and treated with TcES.
- Flow cytometry was used to assess DC maturation markers (CD83, HLA-DR, CD80, CD86).
- Cytokine production (IL-10, IL-1β, TNF, IL-12, IL-6) was measured.
- The expression of C-type lectin receptors (MGL, DC-SIGN) was analyzed.
Main Results:
- TcES did not induce DC maturation markers but enhanced IL-10 production.
- TcES modulated MGL and DC-SIGN expression on DCs.
- TcES significantly down-regulated LPS-induced maturation markers and inflammatory cytokines.
- Pre-exposure of differentiating DCs to TcES amplified the inhibitory effects on LPS response.
Conclusions:
- TcES promotes a tolerogenic-like phenotype in human DCs.
- TcES actively inhibits inflammatory responses mediated by DCs.
- TcES demonstrates potential for immune modulation in helminth infections.
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