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Updated: May 27, 2026
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Published on: May 2, 2017
Evaluation of the magnetic field requirements for nanomagnetic gene transfection
A Fouriki1, N Farrow, M A Clements
1Institute for Science & Technology in Medicine, Hartshill, Stoke-on-Trent, UK.
Unlabelled:
The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency.
Methods:
non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays.
Results:
Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically.
Conclusion:
In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™.
