Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Development of Antibiotic Resistance

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Mechanism of Antibiotic Resistance in MRSA

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...

Antibiotic Selection

Antibiotic Selection

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mediated Electrochemical Probing and Machine Learning for Cysteine and Reduced Monoclonal Antibody Quantification.

Biotechnology and bioengineering·2026

Same author

Genetically-Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Scrambling Signal Modularity in Bottom-up Assembled Synthetic Pseudomonas Consortia Reveals Robust Information Transfer.

ACS synthetic biology·2025

Same author

Electrochemistry as a Tool for Redox-Based Bio-Information Processing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Assessing electrogenetic activation via a network model of biological signal propagation.

Frontiers in systems biology·2025

Same author

Genetically-programmed Hypervesiculation of <i>Lactiplantibacillus plantarum</i> Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: May 27, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

Developing next generation antimicrobials by intercepting AI-2 mediated quorum sensing.

Varnika Roy¹, Bryn L Adams, William E Bentley

¹Graduate Program in Molecular and Cell Biology, University of Maryland, College Park, MD 20742, USA.

Enzyme and Microbial Technology

|November 25, 2011

Summary

This summary is machine-generated.

Antibiotic resistance is a major threat. New antimicrobials targeting bacterial communication (quorum sensing) offer broad-spectrum potential by inhibiting the universal autoinducer-2 (AI-2) signal.

More Related Videos

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

Green Synthesis of Quinoline-Based Ionic Liquid

Green Synthesis of Quinoline-Based Ionic Liquid

Published on: September 27, 2024

Related Experiment Videos

Last Updated: May 27, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

Green Synthesis of Quinoline-Based Ionic Liquid

Green Synthesis of Quinoline-Based Ionic Liquid

Published on: September 27, 2024

Area of Science:

Microbiology
Infectious Diseases
Drug Discovery

Background:

Antibiotic resistance is a growing global health crisis.
Existing antibiotics are becoming less effective against resistant bacterial strains.
New strategies are needed to combat bacterial infections, focusing on virulence inhibition.

Purpose of the Study:

To review emerging strategies for inhibiting bacterial quorum sensing (QS).
To focus on methods targeting the universal autoinducer-2 (AI-2) signaling pathway.
To explore the potential for broad-spectrum antimicrobials derived from AI-2 inhibition.

Main Methods:

Review of current literature on AI-2 signal interception methodologies.
Categorization of inhibition mechanisms based on target sites.
Analysis of AI-2 inhibition strategies against key pathogens like E. coli and P. aeruginosa.

Main Results:

Emerging methodologies effectively intercept the cross-species AI-2 signal.
Inhibition can target signal generation, the signal molecule, or signal transduction.
AI-2 inhibitors show potential for broad-spectrum antimicrobial applications.

Conclusions:

Targeting the universal AI-2 signal is a promising approach for next-generation antimicrobials.
Inhibiting bacterial communication can reduce virulence without strong selective pressure for resistance.
AI-2 interception offers a viable strategy to combat a wide range of bacterial pathogens.