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Updated: May 27, 2026

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
Published on: October 1, 2012
Structural and functional features of Streptolysin O
Ashfaq Ahmad1, Ghosia Lutfullah, Roshan Ali
1Centre of Biotechnology and Microbiology, University of Peshawar, Khyber Pukhtun Khwa, Pakistan. ahmad.biotech@yahoo.com
Abstract:
Streptolysin O, a 63 kDa exotoxin coded by slo gene, is a well-characterised thiol-activated cytolysin, which damages cholesterol-containing membranes resulting in disruption and lysis of the target cell. On the basis of homology model and secondary structure analysis, the toxin has four domains of which domain 4 is of particular importance and is directly linked to domain 2 by a glycine linker and remained consistent in initial membrane recognition. Domain 4 reduces the hydrophobic ratio when compared with its template, which would affect the activity of the toxin at low pH.
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