Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 27, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

A new method to screen polysaccharide cleavage enzymes.

S Badel1, C Laroche, C Gardarin

  • 1Clermont Université, Université Blaise Pascal, Laboratoire de Génie Chimique et Biochimique, Polytech' Clermont Ferrand, 24 avenue des Landais BP 206, 63174 Aubière cedex, France.

Enzyme and Microbial Technology
|November 25, 2011
PubMed
Summary

This study introduces a novel microplate assay for measuring polysaccharide cleavage enzyme activity by detecting changes in viscosity. The method enhances high-throughput screening capabilities for enzyme characterization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical characterization of polysaccharides from Gracilaria gracilis from Bizerte (Tunisia).

International journal of biological macromolecules·2024
Same author

Quantitative structure-property relationship approach to predicting xylene separation with diverse exchanged faujasites.

Physical chemistry chemical physics : PCCP·2018
Same author

Arterial hypertension in patients with atrial fibrillation in Europe: A report from the EURObservational Research Programme pilot survey on atrial fibrillation.

International journal of cardiology·2018
Same author

Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara.

Carbohydrate polymers·2017
Same author

Monovalent and bivalent cations exchange isotherms for faujasites X and Y.

Physical chemistry chemical physics : PCCP·2017
Same author

Pressure dependence of the electrical transport in granular materials.

The European physical journal. E, Soft matter·2017

Area of Science:

  • Biochemistry
  • Enzymology
  • Biotechnology

Background:

  • Traditional methods for evaluating polysaccharide cleavage enzyme activity, such as plate screening, colorimetric, and chromatographic assays, have limitations for high-throughput screening.
  • Advancements in protein engineering necessitate more efficient and sensitive techniques for enzyme activity assessment.

Purpose of the Study:

  • To develop a novel microplate-based method for quantifying polysaccharide cleavage enzyme activity.
  • To overcome the limitations of existing methods in high-throughput screening applications.

Main Methods:

  • A microplate assay was developed utilizing the changes in viscosity of polysaccharide solutions.
  • Polysaccharide solutions were co-incubated with magnetic particles in enzyme buffers, and enzyme activity was measured by the increased mobility of particles in a magnetic field.

More Related Videos

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
06:51

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Related Experiment Videos

Last Updated: May 27, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
06:51

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

  • BioFilm indices (BFI) and associated software were employed to monitor enzyme kinetics and measure key variables.

    • Main Results:

    • The proposed method is sensitive and reproducible, capable of detecting low levels of enzyme activity.
    • The technique effectively measures enzyme kinetics and allows for the evaluation of enzymatic specificity towards different substrates.
    • The viscosity-based assay demonstrates improved efficiency for screening polysaccharide-degrading enzymes.

    Conclusions:

    • The developed microplate viscosity assay offers a sensitive, reproducible, and high-throughput method for evaluating polysaccharide cleavage enzyme activity.
    • This technique addresses the limitations of conventional assays, facilitating enzyme characterization and specificity studies.
    • The method has potential applications in enzyme discovery and optimization within biotechnology and biochemical research.