Related Experiment Video
Updated: May 27, 2026

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Water transport by glucose transporter type 3 expressed in Xenopus oocytes
1Department of Dental Anesthesiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. tomioka@dent.tokushima-u.ac.jp
Abstract:
The hypothesis that the facilitative glucose transporter type 3 (GLUT3) in the brain also exhibits water channel properties similar to that of GLUT1 was tested in Xenopus oocytes expressing human GLUT3 or GLUT1. The volume of oocytes expressing GLUT3 exposed to hypotonic medium increased in an exponential manner. The osmotic water permeability (Pf) for GLUT3 or GLUT1 increased significantly compared with that for oocytes-injected water. The osmotic water transport by GLUT3 was completely inhibited by treatment with a selective GLUT inhibitor, cytochalasin B. The Pf values for GLUT3 significantly increased with increasing temperature of the extracellular medium and the activation energy for GLUT3 was almost the same as that for GLUT1. Thus, GLUT3 in the brain also exhibits water channel properties.
More Related Videos
12:48Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
Published on: February 19, 2013
07:24Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
Published on: December 31, 2016
Related Concept Videos
Transcellular Transport of Solutes
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Membrane Proteins
Glucose Absorption Into the Small Intestine
Secondary Active Transport
Secondary Active Transport