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Related Experiment Videos

Determination of high-density lipoproteins: screening methods compared.

G M Kostner, P Avogaro, G B Bon

    Clinical Chemistry
    |June 1, 1979
    PubMed
    Summary

    This study compared methods for measuring high-density lipoprotein (HDL) cholesterol and apolipoproteins in serum. Both dextran sulfate and phosphotungstate precipitation methods accurately assessed HDL levels, with minimal differences observed.

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    Area of Science:

    • Lipidology
    • Clinical Chemistry
    • Biochemistry

    Background:

    • High-density lipoproteins (HDL) are crucial for cholesterol transport.
    • Accurate measurement of HDL parameters is vital for assessing cardiovascular risk.
    • Hyperlipoproteinemia screening requires reliable analytical methods.

    Purpose of the Study:

    • To evaluate and compare two methods for determining high-density lipoprotein cholesterol (HDL-C) concentrations.
    • To assess the utility of immunochemical quantification of apolipoprotein A-I (ApoA-I) and apolipoprotein A-II (ApoA-II).
    • To optimize laboratory techniques for routine clinical analysis.

    Main Methods:

    • Cholesterol in HDL was measured after precipitating very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) using dextran sulfate or sodium phosphotungstate.

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  • Apolipoprotein A-I and A-II were quantified using Laurell's "rocket" immunoelectrophoresis technique.
  • Immunochemical analysis was performed on both native and delipidated serum samples.

    • Main Results:

    • The dextran sulfate method yielded approximately 5% higher HDL-C values compared to the phosphotungstate method.
    • Both precipitation methods demonstrated excellent correlation (r = 0.95).
    • Immunochemical quantification suggested delipidation might be unnecessary for freshly drawn sera.

    Conclusions:

    • Both dextran sulfate and phosphotungstate methods are reliable for HDL-C measurement.
    • Immunochemical analysis of ApoA-I and ApoA-II is a valuable tool.
    • Streamlining sample preparation for immunochemical assays could improve efficiency.